锶污染土壤中耐锶菌株的筛选

    Screening of Microorganism From Contaminated Soil

    • 摘要: 通过固体培养基平板中锶的梯度变化,从某锶污染土壤中筛选出了26株细菌和20株放线菌。在含锶的液体培养基中,按照细菌对锶的吸附率和耐受性不同,对细菌和放线菌开展了进一步的筛选,并得到了四株对锶有较好耐受性的细菌。四株菌在24 h内的生长倍数超过30倍,对锶的吸附率皆超过20%。它们的最佳生长条件为pH=6.0~7.5、温度为20~40 ℃、锶初始质量浓度在0~2.0 g/L范围内。微生物的16S rDNA鉴定结果表明这四株菌皆为芽孢杆菌属。

       

      Abstract: Through the method of changing Sr concentration in solid medium gradually, 26 strains bacteria and 20 strains actinomyces were isolated from the strontium contaminated soil. Four strains of strontium-resistance bacteria have been selected by the further studies in liquid culture. Magnifications of four bacteria concentration are all more than 30 times and adsorption rates of Sr are more than 20%. The study results show that the bacteria’s optimum growth condition are pH=6.0-7.5, θ=20-40 ℃, ρ0(Sr)=0-2.0 g/L. Identification results show that the microorganism are all subject to Bacillus according to analysis results of 16S rDNA.

       

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