Abstract: Radioactive pollution is increasingly serious, and strontium contamination is a research hot point as one of the typical radioactive pollution. There are some mineralization bacteria in soil which can immobilize the heavy metal ion in the soil. 3 strains was isolated from soil and its characterization and mineralization to Sr²⁺ was carried on. Results show that the removal rate of strains to 1.0 g/L simulated Sr²⁺ is up to 80%. Scanning electron microscope(SEM), energy spectrum analysis(EDS), X-ray diffraction(XRD), Fourier transform infrared spectroscopy(FTIR) results show that mineral products is strontium sulfate. This study demonstrate that using Thiobacillus denitrificans for Sr²⁺ pollution removal in the soil is feasible, and the method will have some application prospect.