Abstract: In order to screen bacteria with strontium tolerance from soil, using flat coating method in beef peptone culture medium to isolate 12 strains from soil. Through the comparative analysis of the Sr²⁺ adsorption effect, 4 strains of bacteria which have good adsorption effect on Sr²⁺ to domestication was selected, and the bacterial growth rate(_υA) before and after acclimation as well as the Sr²⁺ adsorption effect was analysed. The results show that the growth ability of acclimated bacteria in the culture medium containing Sr²⁺ is obviously improved, the peak position(_υA,max) of growth rate (_υA) increases from 1.0 d to 0.5 d. At the same time, the adsorption efficiency of Sr²⁺ is improved after the domestication bacteria. The best adsorption efficiency of screened 4 strains of bacteria on Sr²⁺ can reach more than 90% in the condition of temperature is 30 ℃, rotational speed is 120 r/min, pH is 7 and incubation time is 5 d.