Abstract: A fast and accurate analysis method for the measurement of trace uranium in soil samples was established：microwave digestion-laser fluorescence method. In this method, microwave digestion technique was used to shorten the time of sample pretreatment. The processing speed increases more than ten times. The recovery rate can reach over 95%. Meanwhile, it avoids the problem of cross contamination in the process of multiple samples simultaneously. When the pH value of the sample solution is in the range of 6.5 to 7.5, the fluorescence intensity is the highest, and the UO²⁺₂ and the fluorescence enhancement are the most stable. The measurement sensitivity is the highest. In the process of actual sample measurement, the acidity of sample solution should be adjusted in time, and the acidity of the solution of the standard solution of uranium in the nitric acid system. Fluorescence enhancement agent, sample solution, standard solution and so on are placed in the instrument, until the temperature is stable and then measure on the machine. The apparatus is placed in a relatively stable room, and the optimum temperature is 20-25 ℃. The detection limit is 0.009 μg/g, and the precision of the method is better than 10%(n=9) for 1.4-6.5 μg/g samples.