Abstract: In order to obtain indigenous strains with radionuclide remediation ability in radioactive pollution area in Xinjiang, soil samples were collected for separation of microorganisms. After the pressure tests and adsorption experiments, a filamentous fungus F54 was finally obtained. Through the traditional morphology and molecular biology identification, strain F54 belongs to Fusarium sp., exhibits the highest homology with Fusarium oxysporum strain ATCC MYA-3931. The optimum growth condition is: potato dextrose medium, pH=7.0. The optimum absorption conditions of Cs⁺ for F54 strain are: pH=7.0, 30 ℃, 3-4 d associated with the growth. The Cs⁺ adsorption efficiency of F54 can up to be about 84% tested by ICP-MS. Common cations such as K⁺, Na⁺, Ca²⁺, Mg²⁺, Al³⁺, Fe³⁺ can inhibit the adsorption of Cs⁺. The F54 strain can tolerate 10 kGy dose irradiation of radioactive cobalt, and also has high tolerance of heavy mental ions Ni²⁺, Cr³⁺, Zn²⁺, Co²⁺, Pb²⁺, Hg²⁺. The adsorption efficiency of radioactive ¹³⁷Cs can up to be 62% tested by liquid scintillator. Strain F54 has high potential to repair radioactive pollution in heavy metal contaminated environment.