Abstract: Uranium plays the key role during the development of nuclear energy. But an important issue that has to be solved is the preventability and controllability of uranium pollution. Thus to develop methodologies for uranyl ion detection has attracted much attention. DNAzyme shows great potential in sensors design because of its specific binding activity and exceptional high selectivity. Here, the structure and characters of DNAzyme were introduced briefly. The uranyl ion sensing methods based on DNAzyme developed recently were summarized, including colorimetry, fluorescence, surface-enhanced Raman scattering, resonance light scattering, electrochemistry, and so on. Two enzyme-free amplification techniques that exhibit ultrahigh sensitivity were also recommended. The sensitivity of those based-on DNAzyme methods for uranyl ion detection almost maintains nanomole magnitude. But the performance of the sensors is improved remarkably after combination with enzyme-free amplification techniques. With the developing tendency of sensors for easy fabrication, low cost and portability, it can be expected that DNAzyme-based sensors will have widely application prospect.