Abstract: Solid extraction reagent is the key of separating medical isotope ⁹⁹Tc^m from low specific activity ⁹⁹Mo solution using chromatography. The static adsorption and dynamic adsorption elution experiments have been conducted to study the process and performance of polyamide resin separation ⁹⁹Tc^m from its mother nuclide ⁹⁹Mo. The results show that: polyamide resin can selectively adsorb pertechnetate ions in 10 s, and with 3.85 mg/g saturated adsorption capacity in 3 mol/L NaOH solution. The breakthrough volume of 2.0 g polyamide chromatographic column is about 70 mL, and permolybdate ion remaining on column can be washed by 25 mL 3 mol/L NaOH solution. At last 20 mL water can be used for eluting technetium adsorbed on the polyamide chromatographic column. The research shows that: a novel solid extraction reagent for separation molybdenum and technetium has been developed for automatic ⁹⁹Mo/⁹⁹Tc^m generator. The yield of separation technetium is 98%, and residual concentration of molybdenum is 0.6 mg/L in eluate.