Abstract: In order to obtain the decay data of the short-lived nuclide ¹⁴⁴La, the ¹⁴⁴La samples with high abundance and high activity need to be prepared. In present paper, a separation method was established based on the twice delay separation method, in which the ¹⁴⁴Ba-¹⁴⁴La was separated in 15 s by HDEHP extraction with SISAK separation system, and the newly grown ¹⁴⁴La was separated by P204 extraction chromatographic column at 20 s. The effects of extraction time, concentration of extraction agent and medium condition on the extraction ratio were studied in detail, and the process of rapid radiochemical separation of ¹⁴⁴La from the fission products was established. The process takes about 50 s, and the chemical recovery rate of ¹⁴⁴La is about 75%.