Abstract: The measurement of ⁹⁹Tc in the sample needs to go through pretreatment, separation, and purification processes, which inevitably cause losses of technetium. Therefore, selecting the appropriate tracer is the key to accurately determine the content of technetium. This work adopted the static method combined with column experiment to investigate the separation and purification conditions of ⁹⁹Tc on extraction chromatographic resin(TEVA) and ion exchange resin(AG1-X4), and to explore the behavioral similarities of Re and Tc during chemical separation and purification processes. The results show that TEVA and AG1-X4 resins have strong load capacity for ReO^-₄ and TcO^-₄, the loading condition is in the pH range of 9-11, then the target elements can be eluted with high concentration of HNO₃. The chemical behaviors of Re and Tc are basically the same, and the recovery are all above 90%. Re can be used as a stable non-isotopic chemical analogue of Tc to estimate the recovery yield of ⁹⁹Tc in the analytical process by controlling the experimental parameters.