三氯化铁分光光度法测定药物中亚甲基二膦酸
Spectrophotometric Determination of Methylene Diphosphonate in Pharmaceutical Samples via Complex Formation With Iron(Ⅲ) Chloride
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摘要: 通过三氯化铁与亚甲基二膦酸形成络合物，采用分光光度法，建立了注射用亚锡亚甲基二膦酸盐冻干药盒中亚甲基二膦酸含量的测定方法。结果表明，亚甲基二膦酸质量浓度在0.02~0.08 g/L范围内与吸光度A呈良好线性关系，线性回归方程y=14.61x+0.029 7，线性相关系数r=0.999 1。加标回收率为98.8%~102.0%，相对标准偏差为0.97%(n=6)。该方法可用于快速准确地测定注射用亚锡亚甲基二膦酸盐冻干药盒中亚甲基二膦酸的含量。Abstract: A novel spectrophotometric method for the determination of methylene diphosphonate in a stannous methylene diphosphonate kit via complex formation with iron(Ⅲ) chloride was established. The results show that the absorbance of complex obeys the Lambert-Beer law in the range of methylene diphosphonate mass concentration of 0.02-0.08 g/L, and the linear regression equation is y=14.61x+0.029 7, with the correlation coefficient of 0.999 1. The recoveries are 98.8%-102.0%, and the relative standard deviation is 0.97%(n=6). The proposed method is rapid, simple and sensitive, and therefore can be applied for routine analyses and quality control of methylene diphosphonate in its lyophilized kit.
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Keywords:
- spectrophotometric /
- iron(Ⅲ) chloride /
- methylene diphosphonate
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