Abstract: In this work, a process of systematic separation for Pu and its fission products Pd, Ag, Cd, Sn, Sb and Zr was established. Firstly, these elements in the solution of hydrochloric acid medium were divided into five groups on strong basic anion resin. Then the target elements were separated and purified for each group. The method is simple, rapid and can separate the above 7 elements from the solution of one target. The separation method was verified by using irradiated uranium target. The results show that the chemical yields of the separation process are more than 70% for the 6 fission products, and the decontamination factors of the main interfering nuclides for the gamma spectrometry measurement are more than 1.0×10³. The separation process can meet the requirements of chemical separation for fission yield measurement of valley nuclides from ²³⁹Pu fission.