Abstract: It’s very important of the mutual separation of americium and curium for the accurate measurement of their isotopes by alpha spectrometry and mass spectrometry. However, it is a great challenge to separate Am(Ⅲ) and Cm(Ⅲ) because of their similar chemical properties. This paper made a research on the static adsorption properties and column separation of americium and curium on DGA resin with nitric acid. First of all, the adsorption capacity factor(k′) and separation factor(SF) in different acidities and temperatures were obtained. Next, the parameters such as the peak resolution(R), the theoretical plate height(H) and so on with different column heights and linear flow rates were studied. Based on the chromatographic theories, good separation results that decontamination factors are over 1×10² for both americium and curium when the yields are above 90% have been achieved under the optimal column separation conditions.