Abstract: Research progress of gas chromatography（GC） was reviewed for hydrogen isotope analysis. Related research was included on analysis principle, materials and column design. The main factors of material research include deposit content, cations, and anions. Column design concerns material properties, column type and its length, to meet analysis requirement in various conditions. GC porous layer open tubular(PLOT) columns can be used to analyze ortho/para ration, in which porous alumina is decorated with non-transition metal ions. And columns with porous alumina deposited with transition metal ions, can be used to determine molecules of hydrogen isotopes. The PLOT column is fit for fast analysis, although it has a relative short life; while the packed column has a relative long life, although it has a relative long analysis time. Ordered mesopous materials(OMMs) have potential to improve separation efficiency in cryogenic temperature, and hydrogen isotope elution at room temperature will be accomplished with the aid of hydrogen adsorption catalyzed by transition metal ion such as Cu(Ⅰ).