Abstract: In the preparation of ⁸⁹Zr using a cyclotron to bombard a ⁸⁹Y solid target, trace metal impurities such as Fe, Al, Zn, and Mg, along with unreacted ⁸⁹Y, are present. Separation and purification are necessary for application in ⁸⁹Zr-labeled radiopharmaceuticals. This study conducted the following work: the static distribution coefficients of Zr⁴⁺, Y³⁺, Fe³⁺, Mg²⁺, Zn²⁺ and Al³⁺ on hydroxamate-based resin were measured in hydrochloric acid and oxalic acid systems. The dynamic adsorption rates of these metal ions on the resin column were measured in hydrochloric acid. The breakthrough curves of Zr⁴⁺ in the hydroxamate-based resin were determined in hydrochloric acid. The elution of Zr⁴⁺ and Fe³⁺ from the resin using oxalic acid as an eluent was measured. The purification process conditions were validated using ZrCl₄ simulated solution and trace amounts of ⁸⁹Zr⁴⁺. The hydroxamate-based resin does not adsorb Y³⁺, Al³⁺, Zn²⁺, or Mg²⁺ in HCl. In 2.0 mol/L to 4.0 mol/L HCl solution, the adsorption of Fe³⁺ is poor, while the adsorption of Zr⁴⁺ is good in 2.0 mol/L to 6.0 mol/L HCl solution. The hydroxamate-based resin hardly adsorbs Zr⁴⁺ in oxalic acid solutions above 0.05 mol/L, and its adsorption capacity for Fe³⁺ first increases and then decreases with higher oxalic acid concentrations, reaching the strongest capacity at 0.5 mol/L. Zr⁴⁺ is adsorbed by the hydroxamate-based resin column in 2.0 mol/L to 6.0 mol/L HCl solution, with an adsorption rate exceeding 95%. As the HCl concentration increases to 8.0 mol/L, the adsorption rate drops to 61.42%. Y³⁺, Al³⁺, and Mg²⁺ are hardly adsorbed in 2.0 mol/L to 8.0 mol/L HCl. Fe³⁺ shows negligible adsorption in 2.0 mol/L to 4.0 mol/L HCl, but the adsorption rate increases to 15.69% at 8.0 mol/L HCl. Using 200 mg of hydroxamate-based resin meets the adsorption capacity requirements for Zr⁴⁺ in the purification of ⁸⁹ZrCl₄ solution. During Zr purification, a 0.10 mol/L to 1.00 mol/L oxalic acid solution can be chosen for elution, but it is difficult to wash off Fe with this solution. ⁸⁹Zr mainly concentrates in the elution volume of 400 μL to 1200 μL, with a total recovery rate exceeding 85%. Y³⁺, Al³⁺, Zn²⁺, and Fe³⁺ remain in the adsorption effluent and washing solution, with no impurities adsorbed on the resin column. Zr is almost completely adsorbed on the resin column, and the elution liquid can effectively wash out Zr, with no overlap between Zr elution peaks and impurity peaks. Using hydroxamate-based resin columns, an efficient and rapid method is established for separating and purifying ⁸⁹Zr from Y targets after cyclotron bombardment. Solutions containing Zr⁴⁺, Y³⁺, Fe³⁺, Al³⁺, Zn²⁺, and Mg²⁺ pass through the hydroxamate-based resin columns. Then, 2.0 mol/L HCl and deionized water are used sequentially to wash the columns, and finally, Zr⁴⁺ adsorbed on the resin is eluted with 0.50 mol/L oxalic acid, resulting in a purified oxalic zirconium solution. Studies with stable isotopes and ⁸⁹Zr tracers indicate that the process for purifying ⁸⁹ZrCl₄ solution using hydroxamate-based resin is feasible, and the resin effectively removes Mg²⁺, Fe³⁺, Y³⁺, Al³⁺, and Zn²⁺ from the ⁸⁹ZrCl₄ solution.