用~(111)In标记单克隆抗体和动物分布与显象研究

LABELLING OF McAb WITH ~(111)In AND ITS BIODISTRIBUTION AND IMAGING STUDIES

摘要: 研究了用~(111)In通过DTPA环二酸酐标记单克隆抗体(McAb)的各个实验环节,制备了超纯~(111)In,得到了高比活度1.11GBq/mgMcAb、具有免疫反应活性的~(111)In-McAb。对用~(111)In标记的抗CEA McAb C14-C50、抗人结肠癌McAb 2C10和鼠IgG在荷瘤裸鼠模型体内分布及体外显象进行了研究。注射单抗的动物得到清晰的肿瘤显象,而注射普通IsG的动物的肿瘤不显象。

Abstract: The labelling of McAb with 111In through DTPA bicyclic anhydride has been systematically studied. The 111In-labelled McAb with high specific radioactivity, up to 1.11GBq/mg, without loss of immunoreactivity can be obtained by using ultrapure 111In. The biodistribution and imaging of 111In-labelled anti-CEA McAb C14-C50, anti-colon cancer McAb 2C10 and non-specific mouse IgG in nude mice bearing human colon tumors are investigated respectively. Clear tumor localizations are observed on mice injected with 111In-labelled McAbs, whereas no tumor localization appears in contrast of the tumor-bearing-mice injected with non-specific IgG.