~(211)At偶联CEA单克隆抗体的标记及初步动物实验

LABELLING CEA-McAb WITH ~(211)At AND ITS PRELIMINARY ANIMAL EXPERIMENT

摘要: 以砹苯甲酸为中间体,通过活化羧基反应,制得了~(221)At偶联癌胚抗原(CEA)单克隆抗体(McAb)。改进了文献报道的方法,并采用HPLC对偶联物进行了分析和鉴定。方法全程标记率不低于30％,经凝胶色谱Sephadex G_(75)柱分离得到的偶联物注射液比活度可达3.7×10~4Bq/μg McAb。通过测定~(211)At-CEA McAb和~(211)At-在鼠血液和其它组织中的动态曲线,还讨论了偶联物在动物体内代谢的稳定性。

Abstract: The synthesis of 211At labelled CEA monoclone antibody is performed by an activating reaction of carboxy via para-astato-benzoic interaction. The 211At-CEA-McAb conjugate is separated by a sephadex G75 column and then analysed and identified by hydrophobic interaction HPLC. It contains at least 30% of initial activity of 211At with the specific radioactivity, up to 3. 7×104 Bq/μg McAb. The stability in vivo of the conjugate is discussed by determining dynamic curve in mouse blood and other tissues.