Abstract: Re is prepared via the 186 W(p,n) 186 Re reaction with 16 MeV protons on enriched isotopic 186 W target. Following separation by Al 2O 3 column, 186 Re is concentrated by anion exchange column, and finally the carrier free 186 Re saline solution is achieved. The overall yield of 186 Re is about 85%, with purity of 186 Re >99.2%. The thick target yield of 186 Re is approximately 1.85 TBq/(A·h), and the 186 W recovery is hiqher than 92%.