茶多酚主要成分对肿瘤多药耐药性的逆转作用

茶多酚主要成分对肿瘤多药耐药性的逆转作用

EFFECTS OF TEA POLYPHENOL COMPONENTS ON REVERSION OF TUMOR MULTIDRUG RESISTANCE

摘要

摘要: 将99Tcm MIBI(甲氧基异丁基异腈)和茶多酚(TP)或其主要成分(—) 表儿茶素((—) Epicatechin,EC),(—) 表儿茶素没食子酸脂((—) Epicatechingallate,ECG),(—) 表没食子儿茶素((—) Epigallocatechin,EGC),(—) 表没食子儿茶素没食子酸脂((—) Epigallocatechingallate,EGCG)或咖啡因加入到抗阿霉素人乳腺癌细胞株MCF 7/Adr中进行培养,通过测量细胞对99Tcm MIBI的摄取率来观察茶多酚及其主要成分能否逆转肿瘤细胞的多药耐药性(MDR)。结果表明,茶多酚对MCF 7/Adr细胞的MDR有较明显的逆转作用。茶多酚的4个主要组分的MDR逆转作用顺序为:ECG>EGC>EC>EGCG。但质量比为10∶5∶3∶1的EGCG ECG EGC EC混合物和咖啡因基本上无MDR逆转作用。

Abstract: The effects of tea polyphenol (TP) and its main components,(-)epicatechin (EC), (-)epicatechin gallate(ECG), epigallocatechin (EGC) and (-)epigallocatechin gallate (EGCG) with caffeine on reversion of tumor multidrug resistance (MDR) are investigated by using MCF7/Adr cell line and 99TcmMIBI as a substrate of Pglucoprotein(Pgp). MCF7/Adr cells are incubated with 99TcmMIBI in the presence or absence of TP or its main components (separate or mixed). The cell uptake of 99TcmMIBI is measured and compared with a contrast to estimate the MDR reversion effect.The experimental results indicate that the native tea polyphenol exhibits a moderate MDR reversion effect. The MDR reversion efficacies of the four main components of TP are found in the sequence ECG>EGC>EC>EGCG. Caffeine as well as the mixture of EGCGECGEGCEC in the mass ratio 10∶3∶2∶1 shows practically no MDR reversion effect.

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