Abstract: Subcellular distribution of selenium in cetain important human tissues is studied by neutron activation analysis combined with differential centrifugation method.The standard reference materials,bovine liver NIST 1577a and horse kidney IAEA H8, are used to validate the analytical quality .The experimental results show that the selenium levels in various individuals are significantly differet .Body selenium is highly enriched in human kidney .Different subcelllular selenium distribution patterns are also found in different tissues. The selenium levels in various organelles are sequenced as mitochondria>nuclei>cytosol>lysosome,microsome in liver; nuclei>mitochondria>cytosol>lysosome,microsome in kidney;and mitochondria, nuclei >cytosol,lysosome,microsome in heart.