用生物素-亲和素系统测定放射性标记生物物质的活性
详细信息REATIVITY COMPARISON OF BIOLOGICAL MATERIAL AFTER RADIOLABELING WITH AVIDIN-BIOTIN SYSTEM
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摘要: 分别用131I和188Re标记亲和素,测定其与生物素结合的能力,并与未标记的亲和素、罗丹明标记的亲和素进行比较,测定标记亲和素与生物素结合曲线和半解吸量;探讨用生物素 亲和素系统测定放射性核素标记生物物质活性的可能性。结果表明,未标记的亲和素、罗丹明标记亲和素、188Re 亲和素和131I 亲和素,它们与生物素的结合能力依次下降,与生物素半解吸量分别为21.9,19.5,25.7和47.9μg;放射性标记的亲和素与生物素结合的能力低于未放射性标记的亲和素和罗丹明 亲和素。由此推测,有可能用亲和素 生物素系统来评价标记方法对生物活性的影响。131I和188Re;标记活性Abstract: To find a method for determining the immunoreativity of monoclonal antiboies after radiolabeling avidin is unlabeled and labeled with Rodamine, 131I and 188Re, respectively. The affinities and halfdisorbed amounts of biotin and four kinds of avidin are determined by the biotin columns plus nonlabeled avidin(cold avidin). The affinities of biotin and avidin unlabeled and labeled with Rodamine, 188Re and 131I are decreased in turn. Their halfdisorbed amounts from biotin are 21.9,19.5,25.7 and 47.9 μg of cold avidin. Two kinds of radiolabeled avidin have lower affinity with biotin than that of avidin unlabeled and labeled with Rodamine. There is a possibility to evaluate the reativity of biological materials with different labeling methods by avidinbiotin system.
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Keywords:
- avidin-biotin system /
- radiolabel
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