~(211)At标记抗胃癌单抗3H11及其Fab片段在荷人胃癌裸小鼠体内的分布

~(211)At标记抗胃癌单抗3H11及其Fab片段在荷人胃癌裸小鼠体内的分布

摘要

摘要: 利用ＨＰＬＣ和ＥＬＩＳＡ法对￣（２１１）Ａｔ－３Ｈ１１的分析鉴定表明，间接亲核标记法得到的产品具有３Ｈ１１的天然性和纯度，并保持其免疫活性。￣（２１１）Ａｔ在荷瘤裸小鼠体内分布的结果表明，￣（２１１）Ａｔ－３Ｈ１１Ｆａｂ和￣（２１１）Ａｔ－３Ｈ１１与胃癌细胞有明显的特异亲合力，注射药物３ｈ后，其胃癌摄取率（％／ｇ）分别为９．４８±０．６５和４．９１±２．０５，前者的Ｔ／ＮＴ值在１２－１４ｈ左右明显大于后者。

Abstract: TISSUE BEODISTRIBUTION IN NUDE MICE OF THE α－EMITTER ~(211)At LABELING A MONOCLONAL ANTIBODY 3H11 AND ITS Fab FRAGMENT AGAINST GASTRIC CANCERZHANG SHUYUAN；JIN JIANNAN；LIU NING；WANG JUAN；LUO LIN；WANG FANGYUAN；ZHOU MAOLUN（Institute of Nuclear Science and Technology, Sichuan University, Chengdu, 610064）LUO DEYUAN（Institute of Cancer Research, West China University of Medical Sciences，Chengdu，610041）WAN WENHUI；DONG ZHIWEI（Beijing Institute of Cancer Research,100034 ）ABSTRACT The quality and immunoreactivity of ~(211)At labelled 3H11 are measured by HPLC and ELISAassay， respectively． The biodistribution is performed in nude mice bearing subcutaneous hetero－graft of human gastric cancer cells followling，i．v， injection of ~(211)At－3H11，~(211)At－3H11Fab andNa~(211)At~(211)At,respectively. It is demonstrated that the pharmacokinetics of the astatinated Fab frag-ment appear to be well suited to 7．2h half-life of ~(211)At，since tumor uptake remains high（～10％／g）from 3 to 14h after i．v，injection.Key words~(211)At McAb Biodistribution Human gastric Cancer

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