Abstract: To study the metabolism of 2 15 N Perlolyrine in animals, α 15 N dl tryptophan and 2 15 N perlolyrine are designed and synthesized. α 15 N dl Tryptophan is first synthesized from 15 N glycine via many steps by the use of Al Ni alloy as a catalytic reduction agent, and then 2 15 N perlolyrine is synthesized from α 15 N dl tryptophan.The abundance of 15 N is determined by GC MS, the chemical purity is determined by TLC and the final product is confirmed by MS, UV and TLC. The overall yield of the final product is 7.3%, the abundance of 15 N is 92% and the chemical purity is 98%. The synthetic 2 15 N perlolyrine is suitable for pharmacological experiments.