氚标记胸腺嘧啶核苷的同位素效应

THE ISOTOPE EFFECT ON THE INCORPORATION OF TRITIATED THYMIDBME.

摘要: <正> 淋巴细胞转换率的大小与人体免疫状态密切相关。近来不少报道用氚-胸腺嘧啶核苷(~3H-TdR)掺入法来测定淋巴细胞的转换率,此法的基础是把~3H-TdR和~1H-TdR的物化性质和在生物大分子掺入中的行为看作大体一致而进行示踪研究的。但是由于同位素效应,~3H比~1H的质量数大2倍,因此它们在化学动力学和热力学性质方面有明显的差异,~3H的生

Abstract: In this paper the incorporation of Lymphocyte with 3H-TdR/14C-TdR of different ratio using 14C-TdR as a tracer of Thymidine is described. It is observed that 3H-TdR/14C-TdR before the incroporation is more than twice that after the incorporation.