~(13)CO_2呼气试验的质谱测定方法

DETERMINATION OF ~(13)C IN ~(13)CO_2 BREATH SAMPLE BY MASS SPECTROMETER

摘要: <正> 近十多年来,稳定核素作为一种研究和诊断工具在医学、生物学中的应用进入新的高潮,应用领域不断扩大,技术迅速提高。不少的资料表明,~(13)CO_2呼气试验对一些重要疾病的诊断有较好的结果和较大的潜力,其中用于肝功能测定的呼气试验尤其令人瞩目,很可能发展成为一种新型的临床检验方法,它能直接反映出正常肝细胞的数量和功能。 ~(13)CO_2呼气法是收集人体呼出的CO_2气体,测定~(13)CO_2与~(12)CO_2的同位素比值,根据摄入~(13)C标记化合物前后所采样品之间同位素比值的差异来判断病症和病情。本文报道了采用

Abstract: In this paper, sample collection and handling and isotopic ratio measurement have been reported. The cylinder CO2 is used as the standard gas in the experiment. A vacuum system for preparing the breath test sample is designed. The precision and several relevant factors for MS analysis are discussed.