Abstract: ~(18)F-fluromethylcholine(~(18)F-FCH) as a PET tracer is synthesized.The semi-automatic synthesis assembly of ~(18)F-FCH is modified from CPCU(CTI).The radiochemical purity is measured by analytical HPLC.The radiochemical yield and the radiochemical purity of ~(18)F-FCH are 15% and(>99%),respectively.The total radiosynthesis time is 55 min after EOB.The labeled product exhibited low toxicity.The biodistribution in normal mice and the toxicity are studied.PET imaging with ~(18)F-FCH is performed on tumor xenograft murine model.The semi-automatic synthesis assembly is promising to be used for routine clinic radiopharmaceutial preparation and preliminary study has shown the usefulness of ~(18)F-FCH as an oncologic PET tracer.