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    99Tcm-DTPA-DG标记物的制备

    Preparation Study of 99Tcm-DTPA-DG

      摘要
    • 摘要: 为了在合成二乙三胺五乙酸-脱氧葡萄糖（DTPA-DG）基础上，制备99Tcm-DTPA-DG标记物，以SnCl2·2H2O为还原剂，还原99TcmO-4并与DTPA-DG形成99Tcm-DTPA-DG标记物。进行了DTPA-DG用量、SnCl2·2H2O用量、反应介质pH值、反应温度等对标记率的影响实验。结果表明，25mg DTPA-DG ，500μg SnCl2·2H2O，pH=6，加入Na99TcmO4淋洗液0.5~4mL，在25℃以上放置30min或沸水浴反应10min时，用9g/L NaCl和丙酮作展开剂纸层析法鉴定标记物,放射化学纯度>99%。标记物在室温放置6h，放射化学纯度仍达98.6%。99Tcm-DTPA-DG标记率高，标记物的体外稳定性好，操作简便，便于临床应用。

       

      Abstract: The complex 99Tcm-DTPA-DG is prepared through synthesized DTPA-DG. 99Tcm is used to label diethylenetriaminepentaacetic aciddeoxyglucose ( DTPA-DG ) with stannous chloride reduction. Paper chromatography was performed with acetone and physiological saline as the mobile phase. 99Tcm-DTPA-DG was prepared containing 25mg DTPA-DG and 0.5mg SnCl2. The pH was subsequently adjusted to 6.0. 99TcmO-4 added at room temperature, 30min later, the radiochemical purity of 99Tcm-DTPA-DG is 99%. The radiochemical purity is 986% when it is stable over 6h at room temperature. DTPA-DG can be labeled with 99Tcm easily, efficiently, and with high radiochemical purity, stability and cost effectiveness.

       

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