Abstract: Male SD rats are treated Pb by ip for 25 d, and then fed DMSA through drinking water for 10 d. The levels of lead and several other metal elements in cerebrum, cerebella, hippocampus, liver, kidneys and blood of the rats are measured by X-ray fluorescence method. The lead concentration in cerebrum is higher than that in cerebella and hippocampus. After exposure to lead, Cu, Fe and Mn levels of hippocampus are increased, and Mn in cerebella is decreased significantly (p<0.05), meanwhile Cu, Fe and Zn in kidneys are decreased, and blood Zn and Cu increased (p<0.05). The results show that DMSA could effectively chelate the lead in rat body, the effect of lead on trace elements could be partially eliminated.