Abstract: In order to prepare ⁸⁸Kr samples for the corresponding nuclear data measurement, a method for radiochemical separation of ⁸⁸Kr has been investigated by activated carbon adsorption in this work. The absorption conditions of activated carbon column were studied by using ⁸⁵Kr and ¹²⁵Xe as the radioactive gas tracers. The results show that Xe can be absorbed quickly on the activated carbon column at low temperature (0℃), whereas Kr shows no such property. ⁸⁸Kr samples are easily enriched from the irradiated uranium targets by utilizing the separation system which is designed for the short-lived gaseous fission products. The recovery yield of Kr is better than 90% and the decontamination factors for Xe and I are more than 1×10⁴. The whole procedure can be completed in less than 5 min.