Abstract: The studies on the batch experiments and transport of ⁹⁰Sr and ²⁴¹Am were respectively conducted under unsaturated flow conditions in silty clay column. The results show that the linear correlation coefficient r² of adsorption and desorption isotherms for ⁹⁰Sr and ²⁴¹Am in silty clay medium is greater than 0.9, which indicates that the Freundlich isotherm adsorption model can better describe the adsorption and desorption process of different nuclides in silty clay. The adsorption capacity of strongly adsorbed nuclides ²⁴¹Am is much greater than that of ⁹⁰Sr. As for ⁹⁰Sr, the first-order rate coefficient(named as β) between the dissolved phase and the adsorbed phase was considered in the non-equilibrium adsorption model by transport column. The calculated concentration distribution curve is good agreement with the measured values. The fitting results show that the distribution coefficient(named as K_d) and β of ⁹⁰Sr in the fine sand aquifer are 3.6 mL/g and 0.55/h. Respectively, in terms of ²⁴¹Am, the radionuclides concentration distribution curves in equilibrium adsorption model and non-equilibrium adsorption model are in good agreement with the measured values. It shows that the stronger adsorption capacity of the nuclides have the faster rate of the adsorption-desorption in the soil, meanwhile the shorter time for the system reaction equilibrium. At this time, the fitting results of the equilibrium adsorption mode and the non-equilibrium adsorption model are closer to each other. Meanwhile the calculated K_d value by equilibrium adsorption model is 3×10³ mL/g.