Preparation of ⁹⁹Tc^m(CO)₃-BPHRGD and Biodistribution Evaluation in Normal Mice

⁹⁹Tc^m(CO)₃-BPHRGD was prepared, and the in vitro and in vivo biological evaluations were completed. The labeling yield of ⁹⁹Tc^m(CO)₃-BPHRGD is more than 80% under optimal conditions(pH=7, reacting at 75℃ for 30 min), and the radiochemical purity is more than 98% after purified. Stability in vitro experiments show that the radiolabeled compound shows good stability in physiological saline, fetal bovine serum, and cysteine solution under 37℃. The biodistribution of ⁹⁹Tc^m(CO)₃-BPHRGD in normal mice shows that the metabolic pathways are mainly though liver and kidney with rapid clearance in blood.