JIANG Lin, MU Wan-jun, LIU Guo-ping, XU Yun-shu, LUO Shun-zhong, GAO Qing-xiang. Application of Single Cell Gel Electrophoresis in Post-Evaluation of Organism Radiation Damage[J]. Journal of Nuclear and Radiochemistry, 2009, 31(1): 36-41.
    Citation: JIANG Lin, MU Wan-jun, LIU Guo-ping, XU Yun-shu, LUO Shun-zhong, GAO Qing-xiang. Application of Single Cell Gel Electrophoresis in Post-Evaluation of Organism Radiation Damage[J]. Journal of Nuclear and Radiochemistry, 2009, 31(1): 36-41.

    Application of Single Cell Gel Electrophoresis in Post-Evaluation of Organism Radiation Damage

    • The transient irradiation-caused DNA damage in the human peripheral blood lymphocytes, mouse peripheral blood lymphocytes and alive mouse irradiated by α-ray and γ-ray was investigated, and the single cell gel electrophoresis(SCGE,Comet Assay) was used to detect the extent of DNA damage. On this basis, the dose-effect curve and the evaluating method for radiant after-effect were established, the absorbed dose of alive mouse A irradiated by γ-rays was computed. The results indicate that not only the dose-effect can be described by using SCGE, but also the dose-computed after organism irradiated by radiant rays is achieved with it, and SCGE may be used as a new biological dosimeter.
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