Automatic Synthesis of N-Succinimidyl-4-¹⁸FFluorobenzoate

N-succinimidyl-4-¹⁸Ffluorobenzoate（¹⁸FSFB） was prepared using a multifunction chemistry process control unit module. The dried K₂₂₂/¹⁸F^- was resolubilized with a solution of 4-trimethlammoniumbenzoate trifluoromethanesulfonate and reacted to produce ethyl-4-¹⁸F fluorobenzoate. The ethylester was subsequently hydrolyzed using alkali to obtain the 4-¹⁸F fluorobenzoic acid（¹⁸FFBA）. After purifying through Sep-Pak C18 cartridge, ¹⁸FFBA was dissolved with TSTU in CH3CN and reacted to produce ¹⁸FSFB. The nucleophilic reaction reated for 10 min at 115 ℃ in the covered vial, mixturing solvents with netrogen for several seconds and the ethylester was subsequently hydrolyzed using alkali. The decaycorrected yields of ¹⁸FSFB were much higher. The radiochemical yield of ¹⁸FSFB is (28.2±1.9)% (n=5), and radiochemical purity of ¹⁸FSFB is more than 90%. The whole reaction time is only 45 min from ¹⁸F-F^- to ¹⁸FSFB. The whole reaction time can be shorten and the yield of product is high by using the multifunction chemistry process control unit module. It is a quick and highly efficient method for labeling bioactive compound.