Direct Radiolabeled 99Tcm-EGF and Biological Distribution in C6 Rats[J]. Journal of Nuclear and Radiochemistry, 2008, 30(1): 34-38.
    Citation: Direct Radiolabeled 99Tcm-EGF and Biological Distribution in C6 Rats[J]. Journal of Nuclear and Radiochemistry, 2008, 30(1): 34-38.

    Direct Radiolabeled 99Tcm-EGF and Biological Distribution in C6 Rats

    • The method of technetium-99m direct radiolabeling of epidermal growth factor (EGF) and the biological distribution characteristics of 99TcmEGF in C6bearing rats were explored. Following reduction of EGF(2.5 μg) with 2ME(20 mL/L, 10 μL), EGF was labeled with 99Tcm in the presence of SnCl2(0.5 μg). The results show that radiochemical purity of 99Tcm-EGF is more than 95% and 99Tcm-EGF is of the characteristic of stability. The biological activity analyzed by the culture of endothelial cells shows that there is no significant difference between 99Tcm-EGF and EGF(P>0.05). Biological distribution is observed at different time point after injection of 99TcmEGF into tumorbearing Wistar rats, and shows 99TcmEGF is mainly accumulated in liver, kidney and spleen and least accumulated in the brain. Tumor take relatively high concentration of 99TcmEGF and the uptake ratio of tumor to normal tissue is 2.25 at 4 h. So 99TcmEGF prepared by this direct method appears to be a feasible way to detect the tumors expressing high EGFR(such as glioma).
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