Direct Radiolabeled 99Tcm-EGF and Biological Distribution in C6 Rats
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Graphical Abstract
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Abstract
The method of technetium-99m direct radiolabeling of epidermal growth factor (EGF) and the biological distribution characteristics of 99TcmEGF in C6bearing rats were explored. Following reduction of EGF(2.5 μg) with 2ME(20 mL/L, 10 μL), EGF was labeled with 99Tcm in the presence of SnCl2(0.5 μg). The results show that radiochemical purity of 99Tcm-EGF is more than 95% and 99Tcm-EGF is of the characteristic of stability. The biological activity analyzed by the culture of endothelial cells shows that there is no significant difference between 99Tcm-EGF and EGF(P>0.05). Biological distribution is observed at different time point after injection of 99TcmEGF into tumorbearing Wistar rats, and shows 99TcmEGF is mainly accumulated in liver, kidney and spleen and least accumulated in the brain. Tumor take relatively high concentration of 99TcmEGF and the uptake ratio of tumor to normal tissue is 2.25 at 4 h. So 99TcmEGF prepared by this direct method appears to be a feasible way to detect the tumors expressing high EGFR(such as glioma).
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