THE PREPARATION OF [α-~(32) P]dCTP[J]. Journal of Nuclear and Radiochemistry, 1991, 13(1): 44-44.
    Citation: THE PREPARATION OF [α-~(32) P]dCTP[J]. Journal of Nuclear and Radiochemistry, 1991, 13(1): 44-44.

    THE PREPARATION OF α-~(32) PdCTP

    • Two modifications for Johnson's enzymatic method of preparation of α-32PdCTP are introduced as follows, (1) temperature of reaction in the second stage is controlled at 5-22℃; (2) nuclease P1 is eliminated from the procedure in the third stage. Owing to reduced temperature the final yield of the procedure reaches 90-97% relative to 32Pi. The product cost is reduced and the operating process is simplified as a result of eliminating nuclease P1.
    • loading

    Catalog

      Turn off MathJax
      Article Contents

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return