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THE PREPARATION OF [α-~(32) P]dCTP[J]. Journal of Nuclear and Radiochemistry, 1991, 13(1): 44-44.
Citation: THE PREPARATION OF [α-~(32) P]dCTP[J]. Journal of Nuclear and Radiochemistry, 1991, 13(1): 44-44.
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THE PREPARATION OF [α-~(32) P]dCTP

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  • Received Date: May 29, 1990
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  • Two modifications for Johnson's enzymatic method of preparation of [α-32P]dCTP are introduced as follows, (1) temperature of reaction in the second stage is controlled at 5-22℃; (2) nuclease P1 is eliminated from the procedure in the third stage. Owing to reduced temperature the final yield of the procedure reaches 90-97% relative to 32Pi. The product cost is reduced and the operating process is simplified as a result of eliminating nuclease P1.
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    • References (7)
  • [1]
    Roger,A.J.et al.,United States Patent 4209589,1980．
    [2]
    Symos,R.H.et al.,Methods Emzymol.,29,102(1974) .
    [3]
    Fujimoto,M.et al.,Agric.Biol.Chem.,38,1555(1974) .
    [4]
    Walseth,T.F.,et al.,Biochem.Biophys.Acta,526,11(1979) .
    [5]
    Kenneth,M.,Biochem.J.,131,569(1973) .
    [6]
    Johnson,R.A.et al.,Advances in Cyclic Nucleotide Research,10,163(1979) .
    [7]
    王德宝,祁荣国主编,核酸(上册),北京,科学出版社,1986年,第94-97页。
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