REATIVITY COMPARISON OF BIOLOGICAL MATERIAL AFTER RADIOLABELING WITH AVIDIN-BIOTIN SYSTEM
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Graphical Abstract
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Abstract
To find a method for determining the immunoreativity of monoclonal antiboies after radiolabeling avidin is unlabeled and labeled with Rodamine, 131I and 188Re, respectively. The affinities and halfdisorbed amounts of biotin and four kinds of avidin are determined by the biotin columns plus nonlabeled avidin(cold avidin). The affinities of biotin and avidin unlabeled and labeled with Rodamine, 188Re and 131I are decreased in turn. Their halfdisorbed amounts from biotin are 21.9,19.5,25.7 and 47.9 μg of cold avidin. Two kinds of radiolabeled avidin have lower affinity with biotin than that of avidin unlabeled and labeled with Rodamine. There is a possibility to evaluate the reativity of biological materials with different labeling methods by avidinbiotin system.
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