STUDY ON IODINE LEVELS IN THYROIDS OF IODINE-SUPPLEMENTED RATS BY EPITHERMAL NEUTRON ACTIVATION ANALYSIS
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Graphical Abstract
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Abstract
The second generation female Wistar rats that have been treated with iodinedeficient food,after their delivery,are divided into three groups i.e.excessiveiodine(EI),adequateiodine(AI) and iodinedeficient(ID) according to the KIO3 concentration in the drinking water(3.0,0.4,0 mg/L). In addition,the normal rats with low iodine food and 0.4 mg/L KIO3 water are used as the control group (C).The iodine content in thyroid and the serum thyroid hormone levels of the third generation rats are measured by means of epithermal neutron activation analysis (ENAA),and the method of enzymelinked immunosoerbert assay (ELISA),respectively. The results indicate that the total thyroxine (TT4) and the free thyroxine (FT4) of the EI,compared with those of the controls,are significantly decreased (p<0.001),whereas the free triiodothyronine(FT3) evidently increased (p<0.001).The body weight of the third generation weanling rat of EI is seriously reduced and their thyroid is swollen,but the total iodine content in thyroid is not altered.There is no significant difference between AI and C in the thyroid weight,iodine content and serum hormone levels.This study also indicates that if mother rats suffering iodine deficiency in gestation period gets an adequate iodine intake soon after delivery,an normal iodine level would be found in the body of her baby rat.However,excessive iodine intake may result in low levels of TT4,FT4 and goiter.
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