Labeling of Glycosylated Somatostatin With 99Tcm and Its Biological Evaluation
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Graphical Abstract
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Abstract
Natural somatostatin (SMS), dextran-10(Dx10) and cysteamine were used to synthesis somatostatin-dextran-cysteamine (SMS-Dx10-Cysteamine) conjugate. The in vitrosomatostatin receptor competition binding study was carried out by using 125I-Tyr3-Octreotide as a radioligand to measure the IC50value of SMS-Dx10-Cysteamine. The SMS-Dx10-Cysteamine was radiolabeled with 99Tcm by using gluconate as a transchelator. The radiolabeling conditions of 99TcmCysteamine-Dx10-SMS and its in vitrostability were studied in detail. The biodistribution and blood halflife of 99Tcm-Cysteamine-Dx10-SMS were investigated in normal rats and its tumor uptake and imaging properties were evaluated in nude mice bearing human pancreatic tumor. The results show that SMS-Dx10-Cysteamine remained high receptor binding affinity to somatostatin receptor subtype 2, and the IC50value is in the same range as somatostatin. The radiolabeling efficiency of 99Tcm-CysteamineDx10-SMS is about 85% under optimum conditions and its radiochemical purity is more than 99% after purification. The blood halflife of 99Tcm-Cysteamine-Dx10-SMS in normal rats is 2.38 h post injection. The digestion and excretion is mainly through the hepatobiliary and kidney system. The 99Tcm-Cysteamine-Dx10-SMS is localized in pancreatic tumor and show visible tumor uptake at 6 h imaging. The results indicate that 99Tcm-Cysteamine-Dx10-SMS is a promising imaging agent candidate for somatostatin receptor positive tumor.
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