Radioiodinated of Gambogic Acid and Its Biodistribution in Mice[J]. Journal of Nuclear and Radiochemistry, 2008, 30(1): 39-42.
    Citation: Radioiodinated of Gambogic Acid and Its Biodistribution in Mice[J]. Journal of Nuclear and Radiochemistry, 2008, 30(1): 39-42.

    Radioiodinated of Gambogic Acid and Its Biodistribution in Mice

    • The preparation of radioiodinated gambogic acid and its cell uptake in MCF-7 and biodistribution in mice were investigated. Gambogic acid was labeled with 131I using hydrogen peroxide. The radiolabeled compound was characterized by polyamide TLC, in which the substratum of V(trichoromethane )∶V( methanol ) =40∶1 was used as developing agent. The uptake of 131Igambogic acid in cancer cells was measured. Biodistribution studies were carried out in KM mice. At different time after radiopharmaceutical i.v. administration (185 kBq 131Igambogic acid /mouse), the animals were sacrificed. Blood samples and the tissues of interested were collected, weighted and counted. The percent injected dose per gram (%ID/g) was calculated for each sample. The labeling yield of 131Igambogic acid is 86% and its radiochemistry purity are 97.2%, 95.4%, and 93.3% at 1, 4, and 20 d, respectively. The uptake of 131Igambogic acid in MCF-7 shows markedly higher compared with Na131I(P<0.01).The in vivo biodistribution in mice indicates that 131I gambogic acid is mainly uptaked in liver, kidney, and intestines. The radioactivity in thyroid increases with time. 131Igambogic acid is stable and is markedly uptaked in cancer cells MCF-7. In mice, it is mainly metabolized by liver and excreted through kidney.
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