Radiochemical Synthesis and Receptor Binding Assay of 18F-FDTP as a Putative Dopamine D4 Receptor Imaging Agent
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Graphical Abstract
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Abstract
Imaging of dopamine D4 receptor with specific radioligand has been of increasing interest in recent years. Herein, a putative dopamine D4 receptor tracer, 3-(4-18Ffluorobenzyl)-8, 9-dimethoxy-1, 2, 3, 4-tetrahydrochromeno3, 4-c pyridin-5-one (18F-FDTP) was reportedly prepared from 18F- through a two-step one-pot method. Fluorine-18 was introduced to trimethylammoniumbenzaldehyde triflate by a nucleophilic halogen displacement on it. The fluorine-18 labeled intermediate was then reductively aminated with 8, 9-dimethoxy-1, 2, 3, 4-tetrahydrochromeno3, 4-c pyridine-5-one to form the target molecule. The radiochemical synthesis of 18F-FDTP take about 105 min with a total radiochemical yield of 19.8% (decaycorrected). Its radiochemical purity is greater than 97% after purification via HPLC and its specific activity is greater than 6.3×104 PBq/mol. Through in vitroreceptor binding assay, the Ki of FDTP for D4.2 receptor is determined to be 8.1 nmol/L, and for D3, D2 to be greater than 3 000, 5 800 nmol/L, respectively. The receptor binding experimental results exposed that FDTP demonstrated nanomolar Ki value for binding to the D4 receptor with several hundredfold selectivities towards dopamine D2 and D3 receptors. The lipophilicity of it is determined to be 0.85. In conclusion, 18F-FDTP is radiochemically synthesized and its preliminary in vitrostudies indicate that 18F-FDTP shows promise as a potential imaging agent for dopamine D4 receptor and further studies, such as in vivodistribution and PET imaging studies, are needed to evaluate the new tracer.
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