LI Gu-cai, YIN Duan-zhi, XIA Jiao-yun, CHENG Deng-feng, WANG Ming-wei, ZHENG Ming-qiang, WANG Yong-xian. Radiochemical Synthesis and Receptor Binding Assay of 18F-FDTP as a Putative Dopamine D4 Receptor Imaging Agent[J]. Journal of Nuclear and Radiochemistry, 2008, 30(3): 167-173.
    Citation: LI Gu-cai, YIN Duan-zhi, XIA Jiao-yun, CHENG Deng-feng, WANG Ming-wei, ZHENG Ming-qiang, WANG Yong-xian. Radiochemical Synthesis and Receptor Binding Assay of 18F-FDTP as a Putative Dopamine D4 Receptor Imaging Agent[J]. Journal of Nuclear and Radiochemistry, 2008, 30(3): 167-173.

    Radiochemical Synthesis and Receptor Binding Assay of 18F-FDTP as a Putative Dopamine D4 Receptor Imaging Agent

    • Imaging of dopamine D4 receptor with specific radioligand has been of increasing interest in recent years. Herein, a putative dopamine D4 receptor tracer, 3-(4-18Ffluorobenzyl)-8, 9-dimethoxy-1, 2, 3, 4-tetrahydrochromeno3, 4-c pyridin-5-one (18F-FDTP) was reportedly prepared from 18F- through a two-step one-pot method. Fluorine-18 was introduced to trimethylammoniumbenzaldehyde triflate by a nucleophilic halogen displacement on it. The fluorine-18 labeled intermediate was then reductively aminated with 8, 9-dimethoxy-1, 2, 3, 4-tetrahydrochromeno3, 4-c pyridine-5-one to form the target molecule. The radiochemical synthesis of 18F-FDTP take about 105 min with a total radiochemical yield of 19.8% (decaycorrected). Its radiochemical purity is greater than 97% after purification via HPLC and its specific activity is greater than 6.3×104 PBq/mol. Through in vitroreceptor binding assay, the Ki of FDTP for D4.2 receptor is determined to be 8.1 nmol/L, and for D3, D2 to be greater than 3 000, 5 800 nmol/L, respectively. The receptor binding experimental results exposed that FDTP demonstrated nanomolar Ki value for binding to the D4 receptor with several hundredfold selectivities towards dopamine D2 and D3 receptors. The lipophilicity of it is determined to be 0.85. In conclusion, 18F-FDTP is radiochemically synthesized and its preliminary in vitrostudies indicate that 18F-FDTP shows promise as a potential imaging agent for dopamine D4 receptor and further studies, such as in vivodistribution and PET imaging studies, are needed to evaluate the new tracer. 
    • loading

    Catalog

      Turn off MathJax
      Article Contents

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return