Preparation of 188Re-Lipiodol With Directly Radiolabeling Method
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Graphical Abstract
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Abstract
The aim of this study is to explore the method of radiolabeling lipiodol with 188Re and to appraise the quality of the product. Lipiodol was labeled with 188Re directly at the temperatures from 75 ℃ to 120 ℃ with different reaction conditions. The time-variation labeling efficiency was measured to evaluate the stability of 188Re-lipiodol.The molecular structure change caused by radiolabeling was analyzed with infrared spectrometer. The embedding effect of lipiodol was explored by SPECT imaging of bearing cancer S180 mice. The optimal condition was obtained with the labeling efficiency over (99.1±0.4) %. More than (90.9±1.9)% and (92.8±1.2)% of radioactivity was kept in lipiodol form in room temperature and in 37 ℃ human plasma for 3 d. Infrared spectrum imaging shows that the molecular structure of radiolabeled lipiodol is unchanged. SPECT imaging of S180 tumorbearing mice testifies the radioactivity concentrated in tumor for 48 h after injection of 188Re-lipiodol. The directly radiolabeling method is easy to operate, and the labeling efficiency is higher than (99.1±0.4)%. The radiolabeled product is stable for 72 h, and the embedding effect of lipiodol to 188Re is well. 188Re radiolabeled lipiodol can be used for further investigation.
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