GUO Fei-hu, SHI Ling-li, WANG Ni, WU Ming-xing, ZHANG Yong-ping, DU Jin, ZHANG Lan. Synthesis and Preliminary Biological Evaluation of p-[18F]FSTC[J]. Journal of Nuclear and Radiochemistry, 2010, 32(4): 236-242.
    Citation: GUO Fei-hu, SHI Ling-li, WANG Ni, WU Ming-xing, ZHANG Yong-ping, DU Jin, ZHANG Lan. Synthesis and Preliminary Biological Evaluation of p-[18F]FSTC[J]. Journal of Nuclear and Radiochemistry, 2010, 32(4): 236-242.

    Synthesis and Preliminary Biological Evaluation of p-[18F]FSTC

    • Carbonic anhydrase Ⅸ (CA Ⅸ) is a promising target for tumor hypoxia imaging, and some sulfamide compounds can specifically bind to human CA Ⅸ. In order to explore a novel positron emission tomography(PET) probe for detecting CA Ⅸ, the 1-(2-[18F]fluoroethyl)-N-(4-sulfamoylphenyl)-1H-1, 2, 3-triazole-4-carboxamide(p-[18F]FSTC) which has specific binding affinity to CA Ⅸ was radio-synthesized fast and efficiently by a “click chemistry” method. The labeling efficiency of p-[18F]FSTC is 90% and the total synthesis time is about 60 min. The radiochemical purity of p-[18F]FSTC is over 98% after HPLC purification. The stability of p-[18F]FSTC both in vitro and in vivo is high. The biodistribution of p-[18F]FSTC in normal mice shows that the clearance of p-[18F]FSTC in the blood is very slow; the uptake in spleen, lung, kidney and stomach is high. These preliminary results provide some experimental basis for further study of fluorine-18 labeled sulfamide as PET probes for tumor hypoxia imaging.
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